| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.066759 |
| Chromosome: | chromosome 2 |
| Location: | 2759523 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g093950 | PUMPKIN | (1 of 1) 2.7.4.22 - UMP kinase / Uridylate kinase; Putative plastid UMP kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGTGCGTGTACGGCGGCACAAATCGCATCCAAATGCCGAACAGCTAGG |
| Internal bar code: | AATTTTCTACGCATGGACGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2718 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGTACAATTAGCGTGCCG |
| Suggested primer 2: | ATCACGCTGTGCAAGGAGAA |