Insertion junction: CLIP2.066763_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.066763
Chromosome:chromosome 7
Location:3977288
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g339850 (1 of 18) 2.7.10.2//2.7.11.1 - Non-specific protein-tyrosine kinase / Cytoplasmic protein tyrosine kinase // Non-specific serine/threonine protein kinase / Threonine-specific protein kinase 3'UTR
Cre07.g800824 (1 of 352) PF00078 - Reverse transcriptase (RNA-dependent DNA polymerase) (RVT_1) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGCAGCAAAGTCGGGGTTGGGCCCTGATAGCATGTGATGGCTCGGCGTC
Internal bar code:GCTTTCTTTCGTGATCCCCTAA

Confirmation - LEAP-Seq

LEAP-Seq distance:1629
LEAP-Seq percent confirming:15.5556
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:38
LEAP-Seq n unique pos:45

Suggested primers for confirmation by PCR

Suggested primer 1:GAACATTGCAACTGCGCTGA
Suggested primer 2:TGGTTTTGGGACTGCACTGT