Insertion cassette: | CIB2 |
Side of cassette: | 3' |
Strand: | - |
Strain: | CLIP2.066763 |
Chromosome: | chromosome 7 |
Location: | 3977288 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre07.g339850 | (1 of 18) 2.7.10.2//2.7.11.1 - Non-specific protein-tyrosine kinase / Cytoplasmic protein tyrosine kinase // Non-specific serine/threonine protein kinase / Threonine-specific protein kinase | 3'UTR | |
Cre07.g800824 | (1 of 352) PF00078 - Reverse transcriptase (RNA-dependent DNA polymerase) (RVT_1) | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCAGCAAAGTCGGGGTTGGGCCCTGATAGCATGTGATGGCTCGGCGTC |
Internal bar code: | GCTTTCTTTCGTGATCCCCTAA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1629 |
LEAP-Seq percent confirming: | 15.5556 |
LEAP-Seq n confirming: | 7 |
LEAP-Seq n nonconfirming: | 38 |
LEAP-Seq n unique pos: | 45 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GAACATTGCAACTGCGCTGA |
Suggested primer 2: | TGGTTTTGGGACTGCACTGT |