| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.066765 |
| Chromosome: | chromosome 9 |
| Location: | 975156 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g402600 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCAACATGGCGCAAGTACTACTCAGCCGCAGCAGGCTGCTGCTTCTAAG |
| Internal bar code: | TGTTTAACTTCTTGAGATGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2021 |
| LEAP-Seq percent confirming: | 88.5714 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGGCAGAGGTGGAGACAG |
| Suggested primer 2: | TCGGGAATCGTACCAGACCT |