| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.066777 |
| Chromosome: | chromosome 6 |
| Location: | 373112 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g251500 | SPS1 | (1 of 1) K18787 - thermospermine synthase (ACL5); Spermine synthase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTACTGGTAGGCGCCAACGCGCCCCCATCAGGTGACTCCCACGGGTGGCT |
| Internal bar code: | GCTAGCGACGGGGATACGGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 849 |
| LEAP-Seq percent confirming: | 83.3333 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTAGAGCTGTTGCGCTTGA |
| Suggested primer 2: | GTTCGTGGGATGCTTTGCTC |