| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.066780 |
| Chromosome: | chromosome 13 |
| Location: | 1864935 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g575366 | 3'UTR | ||
| Cre13.g575400 | (1 of 33) IPR013830 - SGNH hydrolase-type esterase domain | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAACCGCTTAAACCCAAACGCCAGCCCCAACATCCAGCAGCAAACGAAC |
| Internal bar code: | ATCCGGCTTGAATGGGCTATCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5369 |
| LEAP-Seq percent confirming: | 71.4286 |
| LEAP-Seq n confirming: | 45 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 63 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATAGTGACCTACAGGCCGGT |
| Suggested primer 2: | TGCGTCCCTTGTCATCAGAC |