| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.066789 |
| Chromosome: | chromosome 14 |
| Location: | 3973667 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g632950 | NOT1 | (1 of 1) K12604 - CCR4-NOT transcription complex subunit 1 (CNOT1, NOT1); Component of CCR4-NOT transcriptional regulator complex | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAGTCCCGGATGTTTTATCGCGTCCCGAGTGTGCGAGTCCCAGTGTAGT |
| Internal bar code: | GCTGGAGTTATTAGATAGCAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2639 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 42 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGGTAGGGTAGGCTCACT |
| Suggested primer 2: | GGCCTGCTCATCACCTTCAT |