| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.066818 |
| Chromosome: | chromosome 2 |
| Location: | 7227777 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g390949 | FAP140 | Flagellar Associated Protein 140 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATACCATATCATGTCAATTCGGGTGATGCGTATGGTGTCATGTGGTTCC |
| Internal bar code: | GAGAGACCCGTGTGTGTCCGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2105 |
| LEAP-Seq percent confirming: | 54.6296 |
| LEAP-Seq n confirming: | 59 |
| LEAP-Seq n nonconfirming: | 49 |
| LEAP-Seq n unique pos: | 108 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATGGTGACCTTCGAGCCAA |
| Suggested primer 2: | AGACACCATGAATGCCTGGG |