| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.066825 |
| Chromosome: | chromosome 6 |
| Location: | 6607508 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g294550 | (1 of 1) IPR000104//IPR008537 - Antifreeze protein, type I // Protein of unknown function DUF819 | 3'UTR | |
| Cre06.g294600 | (1 of 3) PF15227 - zinc finger of C3HC4-type, RING (zf-C3HC4_4) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCCCCATAGTGCAATGCGCCCCACACTCAGTCTTCAGTCCTTGCCACA |
| Internal bar code: | CTGCCTTTGCTATTGATATATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1006 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGTGTAGCTGGCTGTGTA |
| Suggested primer 2: | AATAGGTCGCAGCAGATCGG |