| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.066830 |
| Chromosome: | chromosome 1 |
| Location: | 6071262 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g042900 | RRM1 | Putative rRNA (adenine-N6-)-methyltransferase; (1 of 1) 2.1.1.183 - 18S rRNA (adenine(1779)-N(6)/adenine(1780)-N(6))-dimethyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCCGCCACCCCCTCCTGCCTCCTGCCACTCATATTCTCCCCCCCTTTG |
| Internal bar code: | TCTTAAGATACGTGATCGGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 140 |
| LEAP-Seq percent confirming: | 71.4286 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCAGGCTAGCGTGTCTAG |
| Suggested primer 2: | CGTCTTGTTTTTGCGGCTGA |