| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.066833 |
| Chromosome: | plastome |
| Location: | 125809 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| CreCp.g802315 | psbI,ChreCp051,2717042 | photosystem II protein I; (1 of 1) K02710 - photosystem II PsbI protein (psbI) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATACAAAGTAATATTAAAGCCAATTAAAGAAAAAAACAATGTAATATCT |
| Internal bar code: | CTTCGAAGCAGAAATGTCCATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 194 |
| LEAP-Seq percent confirming: | 80.0 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTAGCTCGTGGTGCTCGTTT |
| Suggested primer 2: | TGCACGGTAGGTATAGGGCA |