Insertion junction: CLIP2.066849_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.066849
Chromosome:chromosome 17
Location:763044
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g701200 RPL14 Cytosolic 80S ribosomal protein L14; (1 of 1) K02875 - large subunit ribosomal protein L14e (RP-L14e, RPL14) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTTTAGAGTGGCAACGATGGTCCGTGGTGTGACGGGCTGGAGCATTCGG
Internal bar code:GGTGTTCACGCACTGGGCCGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:3667
LEAP-Seq percent confirming:98.4127
LEAP-Seq n confirming:62
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:63

Suggested primers for confirmation by PCR

Suggested primer 1:AGGTGCGAGTGAGAAGTTGG
Suggested primer 2:CGCTTCTTGTAGCATCACGC