| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.066881 |
| Chromosome: | chromosome 14 |
| Location: | 1298759 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g616450 | CYG46 | (1 of 2) PF00211//PF03924 - Adenylate and Guanylate cyclase catalytic domain (Guanylate_cyc) // CHASE domain (CHASE); Adenylate/guanylate cyclase | 3'UTR |
| Cre14.g801612 | (1 of 352) PF00078 - Reverse transcriptase (RNA-dependent DNA polymerase) (RVT_1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAGGGAACCTCCATCACCCACCTGTCATCGACCCATCTCCGGAGGCAGC |
| Internal bar code: | GGACATAGACCATAATATGAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1870 |
| LEAP-Seq percent confirming: | 96.7742 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTTGTCAACAGAACGCAC |
| Suggested primer 2: | TTGCGCATCCCTTCATGTCT |