| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.066893 |
| Chromosome: | chromosome 4 |
| Location: | 2653092 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g223100 | CAH1 | (1 of 2) PTHR18952//PTHR18952:SF104 - CARBONIC ANHYDRASE // CARBONIC ANHYDRASE-RELATED PROTEIN; Carbonic anhydrase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTGGGGCGCTTCAGCACGGTATGGTTGCCAGCATGCACGGTCCGGCCT |
| Internal bar code: | TAATTTAAGTAGGTCGGTATGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1070 |
| LEAP-Seq percent confirming: | 93.3333 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCCCACGTTTTCCCTTTG |
| Suggested primer 2: | GCTTCACGGTTTGGTGTGTC |