| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.066917 |
| Chromosome: | chromosome 10 |
| Location: | 4077810 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g448150 | (1 of 6) PF01847 - von Hippel-Lindau disease tumour suppressor protein (VHL) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACTGGGGGCGCCGCTGAGGCTCCGAGCAACTGTACTACGAGGACGTGAC |
| Internal bar code: | CGCCATTGATGTAGCTACTGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3193 |
| LEAP-Seq percent confirming: | 38.9262 |
| LEAP-Seq n confirming: | 58 |
| LEAP-Seq n nonconfirming: | 91 |
| LEAP-Seq n unique pos: | 149 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGATTGCCTAACTTGCCCCC |
| Suggested primer 2: | CCGGTTCTGTGGCAGTACAT |