| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.066918 |
| Chromosome: | chromosome 6 |
| Location: | 706176 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g254600 | MRPS45,mS45 | Mitochondrial ribosomal protein S45 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCTACCGTAACCCTGCTGCTCCTCGTCCCTAGGTCCATATTCGCGCGTC |
| Internal bar code: | TAAACATTGAGGAAGTAGGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 516 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGGGGTAGAGGGGAGAAG |
| Suggested primer 2: | CATCATCAAGCGCCAGAAGC |