Insertion junction: CLIP2.066920_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.066920
Chromosome:chromosome 1
Location:5659694
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre01.g040450 HDT1,HDPT1 Histidine-aspartic acid phosphotransferase; (1 of 1) K14490 - histidine-containing phosphotransfer peotein (AHP) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCAGCTGCGCCAGCAATGTGTCAATGCCCTGGCACACAGCTGCATATTGA
Internal bar code:GGTATTTGCGAGTGTCGAGGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:662
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CAGCCTACTACCGCTGTACG
Suggested primer 2:TGCTGCTCTAACGCCATGAA