| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.066925 |
| Chromosome: | chromosome 9 |
| Location: | 2953841 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g390150 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGACAAGGAGCGGCCTGCTGCGGCTCGGCCGGACCGGAGCCAAACGCA |
| Internal bar code: | TCTGAGGGAGGGAGGTTATTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 152 |
| LEAP-Seq percent confirming: | 1.69492 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 58 |
| LEAP-Seq n unique pos: | 59 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGAACAAGCTGTCCACCG |
| Suggested primer 2: | ACTGTGCTCACGCTCGTTTA |