Insertion junction: CLIP2.066926_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.066926
Chromosome:chromosome 12
Location:5126361
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g801425 (1 of 1) 2.7.10.2//2.7.11.17 - Non-specific protein-tyrosine kinase / Cytoplasmic protein tyrosine kinase // Calcium/calmodulin-dependent protein kinase / Microtubule-associated protein 2 kinase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGAGAACAGTGGCTCAACTGGGGACGCCATGCACCCACAGCAGCTGCAG
Internal bar code:AGCCAGGGGCTCGGTTTGTTAC

Confirmation - LEAP-Seq

LEAP-Seq distance:1073
LEAP-Seq percent confirming:4.34783
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:66
LEAP-Seq n unique pos:69

Suggested primers for confirmation by PCR

Suggested primer 1:TCGAGTGTGAGCTGTGTGAC
Suggested primer 2:CATGGCGCTCTTCATCAAGC