| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.066927 |
| Chromosome: | chromosome 12 |
| Location: | 1587606 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g487150 | PGM20 | (1 of 2) PTHR23029//PTHR23029:SF24 - PHOSPHOGLYCERATE MUTASE // SUBFAMILY NOT NAMED; Putative phosphoglycerate mutase | 3'UTR |
| Cre12.g487200 | (1 of 5) IPR001680//IPR011047//IPR017986//IPR020472 - WD40 repeat // Quinonprotein alcohol dehydrogenase-like superfamily // WD40-repeat-containing domain // G-protein beta WD-40 repeat | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGTTTTAGCGGCCTTGGCCAGGCGCGTTTGCAACTAACAGCAGAGTGAG |
| Internal bar code: | AGCTCATAAATGAGCGACAACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2726 |
| LEAP-Seq percent confirming: | 93.6508 |
| LEAP-Seq n confirming: | 59 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 63 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGGCTGTGGCAAGATACC |
| Suggested primer 2: | GCGCTTCTGTTCCCTACCTT |