| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.066931 |
| Chromosome: | chromosome 3 |
| Location: | 293767 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g144444 | (1 of 1) IPR022416 - Prion/Doppel protein, beta-ribbon domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGTTGGACATGACGGATGGGTAACCGAATGACGAACACATGACCCTGAA |
| Internal bar code: | GGTAAGTTGCCCGAGCTTACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1883 |
| LEAP-Seq percent confirming: | 96.2963 |
| LEAP-Seq n confirming: | 78 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 81 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACCCTGGCTAGCGTGTAC |
| Suggested primer 2: | AGCAGAAGGAGGAGGAGGAG |