Insertion junction: CLIP2.066938_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.066938
Chromosome:chromosome 17
Location:6529596
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g744797 (1 of 1) PF05623 - Protein of unknown function (DUF789) (DUF789) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGCACACAAACCCAAACACCGTTTCCCAGCTACTCTTCTCTCTTAGCATG
Internal bar code:TATCGGTCTCCTGGAGTAAGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:2162
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:47
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:47

Suggested primers for confirmation by PCR

Suggested primer 1:GCGCGAGATAACAGCACTTG
Suggested primer 2:ATGTTTCGTTTTGCGCACGA