| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.066943 |
| Chromosome: | chromosome 7 |
| Location: | 4989584 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g347200 | (1 of 1) 1.8.4.14 - L-methionine (R)-S-oxide reductase / FRMsr | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGAAAACACGCCCGCACCGTCCCACACTTTCCCACACCTTTTCACACCT |
| Internal bar code: | TATCACGAGTGTCCTTAAGCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2348 |
| LEAP-Seq percent confirming: | 2.5 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 39 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAAGCACACGCACTAGGCAA |
| Suggested primer 2: | ACTGTACCAATGCGCAGACA |