Insertion junction: CLIP2.066944_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.066944
Chromosome:chromosome 8
Location:290369
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre08.g358568 (1 of 14) PF04577 - Protein of unknown function (DUF563) (DUF563) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAACGTGATTGTGTCGTACGACCCCGAGACGCACCCCAAACACAGCATGG
Internal bar code:CAAACTGTAGCACGAACAGAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:379
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:AAGTCGTCAGCAGTTCAGGG
Suggested primer 2:AGGAGCAGGCTAGCAAACTG