| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.066945 |
| Chromosome: | chromosome 6 |
| Location: | 7211237 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g298750 | AOT4 | (1 of 9) PF01490 - Transmembrane amino acid transporter protein (Aa_trans); Amino acid transporter | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTCAGGTACACGTGAGGAGGTGTGACTAATGAACAACGGATCAGGCAG |
| Internal bar code: | GCACTCAAGGGGAACCGAGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1435 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGCAAACGGGTTTTCCTC |
| Suggested primer 2: | CTGCAGTCGTACCTCTAGCG |