| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.066952 |
| Chromosome: | plastome |
| Location: | 73099 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| CreCp.g802293 | orf202 | Wendy transposon | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCGTAGGATAGTGATAATGTCACTGCCCTGACCCCCTGGCACATCCCA |
| Internal bar code: | GATGCGCCGTTATGGCCTGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 340 |
| LEAP-Seq percent confirming: | 80.0 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAAAACACAAGCTGCACCGC |
| Suggested primer 2: | AGCATGCTATGGTTGGCTGT |