| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.066955 |
| Chromosome: | chromosome 10 |
| Location: | 3866189 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g446200 | (1 of 1) PF11566 - PI31 proteasome regulator N-terminal (PI31_Prot_N) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAAAAGTTACCGCCGTGACAGGGCGCTCGACCGGTGCGCCACCACCAGC |
| Internal bar code: | GGCCGGTCATCATGCTTTGTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 6478 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 107 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 107 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGTTTCAGGGCACAACTGT |
| Suggested primer 2: | ATACATGGGCGCATAGTCGG |