| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.066956 |
| Chromosome: | chromosome 3 |
| Location: | 6115616 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g190550 | (1 of 1) PF08638 - Mediator complex subunit MED14 (Med14) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACCTATACTGAACTGTCCACTAAAGAAGTAAGCAGAAAGCCAGGATAAA |
| Internal bar code: | GGGAACGAGTGGTTGAGGCAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1619 |
| LEAP-Seq percent confirming: | 94.2857 |
| LEAP-Seq n confirming: | 33 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCACGGTGAATTAATCCGC |
| Suggested primer 2: | TGGGCCGGAGCAAATTATGT |