Insertion cassette: | CIB2 |
Side of cassette: | 3' truncated? |
Strand: | + |
Strain: | CLIP2.066958 |
Chromosome: | chromosome 2 |
Location: | 5976225 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre02.g118300 | RHE,HEL12 | (1 of 1) PTHR24031:SF330 - DEAD-BOX ATP-DEPENDENT RNA HELICASE 7; DEAD box ATP-dependent RNA helicase | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTACCGAACGGGCAATGAGTGCGGAAAGCAGCAGCTGCATTAGGGCGA |
Internal bar code: | TCTGTGAAACTGAGGAGGTCGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 2743 |
LEAP-Seq percent confirming: | 85.7143 |
LEAP-Seq n confirming: | 6 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TTCTGGTAGTCCCACGCAAC |
Suggested primer 2: | CTATGGTTTTGTGCGGCTGG |