| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.066977 |
| Chromosome: | chromosome 16 |
| Location: | 4833679 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g687350 | ACO3,ACX3 | (1 of 1) PTHR10909:SF11 - ACYL-COENZYME A OXIDASE-LIKE PROTEIN; Acyl-CoA oxidase/dehydrogenase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAATTTATAGCGTCGCCATTAGGACGCGACAAATCTTCTTGTGTGGTACG |
| Internal bar code: | CCAGGCCTAGATTCAGAAGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4591 |
| LEAP-Seq percent confirming: | 97.8495 |
| LEAP-Seq n confirming: | 91 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 93 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAACACTAACGCACGTGCA |
| Suggested primer 2: | GTGTAGAGGGCTGGAGGAGA |