| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.066981 |
| Chromosome: | chromosome 2 |
| Location: | 227879 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g800153 | 5'UTR | ||
| lncRNA_TCONS_00054465 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGAGGCCCATGGGCGGAGGGGTCGCAGCTGCCATGGAGCTGCAGAACA |
| Internal bar code: | ATAGGGGCAAGCTTGGATGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4889 |
| LEAP-Seq percent confirming: | 99.2593 |
| LEAP-Seq n confirming: | 134 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 135 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGGTTTGCAGCAAAGTCG |
| Suggested primer 2: | GCCTTGTCACGTTCCCAAAC |