| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.066991 |
| Chromosome: | chromosome 9 |
| Location: | 5738318 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g410650 | HIS1 | (1 of 1) 2.4.2.17 - ATP phosphoribosyltransferase / Phosphoribosyl-ATP pyrophosphorylase; N-5-phosphoribosyl-ATP transferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAATGTACCGTATGTCTGTAAGCGCCCCCAGAAGTCTCACATTACCGGGA |
| Internal bar code: | GAATACTCTTCGTGCTGTAAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1286 |
| LEAP-Seq percent confirming: | 97.1429 |
| LEAP-Seq n confirming: | 34 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAAGTGGTTGATTGGGCGC |
| Suggested primer 2: | TACGGCCTTACATGAGGGGA |