| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.066992 |
| Chromosome: | chromosome 17 |
| Location: | 281942 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g697850 | (1 of 226) IPR012337 - Ribonuclease H-like domain | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAACTAGACACCGCTCCCGACTAGGGGGCAAGTTGGCATGCGGAGGTGG |
| Internal bar code: | CGCGCGCGTTTTGTGCAAACTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2199 |
| LEAP-Seq percent confirming: | 16.6667 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGGTTGTGCGAAACAGTG |
| Suggested primer 2: | ACTGAACCACTCGCACTCAG |