| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.066998 |
| Chromosome: | chromosome 17 |
| Location: | 5787049 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g741200 | EB1,EBP1 | (1 of 1) K10436 - microtubule-associated protein, RP/EB family (MAPRE); Microtubule plus-end binding protein EB1 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGAAGTGAGATGTGCTGCCAGCATTTTTCTGGACCCCGTGCACGGCGCA |
| Internal bar code: | GTTTCAAGAATGCCATGAGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5239 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 46 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTATTGGCTGATGCGCAGG |
| Suggested primer 2: | TCCTTACAGGATGCAGCACG |