| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.067004 |
| Chromosome: | chromosome 5 |
| Location: | 2550465 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g239400 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTATGTACGCCACCGCATCCTTCCCCCGTCCTCAAAGCGCCACGCGCCAG |
| Internal bar code: | GATTACACGACCGGATTTTAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2300 |
| LEAP-Seq percent confirming: | 97.8261 |
| LEAP-Seq n confirming: | 45 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGTGCTGCTGTCATTGGC |
| Suggested primer 2: | TGTAGCCCAGTAGCCCTCAT |