| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.067012 |
| Chromosome: | chromosome 12 |
| Location: | 95424 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g484000 | BCX1,ACX1 | Beta-carboxyltransferase (ACCase complex); (1 of 1) K01963 - acetyl-CoA carboxylase carboxyl transferase subunit beta (accD) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGAGAGCAGAATGATGAGACTACTTCGCCACCGCCCACAAGGACATCCC |
| Internal bar code: | ACGTTCTAGTGGGTTTTTCATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3037 |
| LEAP-Seq percent confirming: | 58.8235 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 51 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCCATCCAAGTGCGTTACA |
| Suggested primer 2: | GGTGGTGGTAGTGAGGTTGG |