Insertion junction: CLIP2.067018_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.067018
Chromosome:chromosome 9
Location:6673559
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g416700 Related to rhamnosyl methyltransferase; (1 of 10) PF13578 - Methyltransferase domain (Methyltransf_24) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGAAGTTGGCCAAGATACCTGCTTGGCATGCACCATGCCGGCCCATGCA
Internal bar code:TATTTTGCGTTTCCCTGACCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:2206
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:54
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:54

Suggested primers for confirmation by PCR

Suggested primer 1:TGCCTTGACACTGTCCCATC
Suggested primer 2:CGTTGGCGAAGTCTGTAGGT