| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.067020 |
| Chromosome: | chromosome 16 |
| Location: | 4035951 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g690130 | CDS | ||
| Cre16.g690150 | ALG6,GTR21 | (1 of 1) 2.4.1.267 - Dolichyl-P-Glc:Man(9)GlcNAc(2)-PP-dolichol alpha-1,3-glucosyltransferase / Dol-P-Glc:Man(9)GlcNAc(2)-PP-Dol alpha-1->3-glucosyltransferase; Alpha-1%252C3-glucosyltransferase 6 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTCGATCCTAGCCGTGCGAACACAACCGTAGATACGCGCGGAACGCAGC |
| Internal bar code: | ACATTCGGTTAGCGAGGATGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1459 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTAGACGTGTGAGGTGCA |
| Suggested primer 2: | GATGACGCACATGACGATGC |