| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.067021 |
| Chromosome: | chromosome 9 |
| Location: | 3575338 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g396661 | DUO3,DIV39 | (1 of 2) IPR000104//IPR001005//IPR009057 - Antifreeze protein, type I // SANT/Myb domain // Homeodomain-like | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACCTCTCCCGATGCCTCCCATCGCGAACCCGAGCCTGAGCAATGCACTG |
| Internal bar code: | AGAAGCTCGCGAGTTGGGATCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 782 |
| LEAP-Seq percent confirming: | 81.25 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCATCCGCCAGTTCCAGAA |
| Suggested primer 2: | TCAGTCTGGGGAATGGCAAC |