| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.067034 |
| Chromosome: | chromosome 8 |
| Location: | 4355226 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g384800 | (1 of 2) 3.1.1.81 - Quorum-quenching N-acyl-homoserine lactonase / Quorum-quenching N-acyl homoserine lactone hydrolase | 3'UTR | |
| Cre08.g384850 | SDR16 | Short-chain dehydrogenase/reductase; (1 of 5) 1.3.1.38 - Trans-2-enoyl-CoA reductase (NADPH) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGGAGGGCCGGGTGCTGTGTAACAAGTGAGGAGTGCGCGAGGACTGGC |
| Internal bar code: | GATGTTAGAGTCGGTGTCGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 839 |
| LEAP-Seq percent confirming: | 66.6667 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCAACGGCCAGGTGTAAGG |
| Suggested primer 2: | CAGGCGTGTGTCTTTGTTGG |