| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.067038 |
| Chromosome: | chromosome 9 |
| Location: | 3053468 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g393802 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACTTCTTTTGACCGGCCGGCCAGACAGGTCCGCAGCGGTTGCTGCAAGC |
| Internal bar code: | ATTTATGGGTTCGACGGGTTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3893 |
| LEAP-Seq percent confirming: | 56.9892 |
| LEAP-Seq n confirming: | 53 |
| LEAP-Seq n nonconfirming: | 40 |
| LEAP-Seq n unique pos: | 93 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAATAAGCCACGTCCCCTGT |
| Suggested primer 2: | CTGCGAAAGTTGTGTGTCCG |