| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.067043 |
| Chromosome: | chromosome 2 |
| Location: | 6982524 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g389430 | TSF3 | (1 of 1) PTHR11538:SF40 - PHENYLALANINE--TRNA LIGASE ALPHA SUBUNIT; Phenylalanyl-tRNA synthetase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGATAACTGCAGTTTGGTGGCGTGTGGCTGGAGGTGTTGGCGATGGAGT |
| Internal bar code: | GCTGGACTAACATTGTTACCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1864 |
| LEAP-Seq percent confirming: | 97.2222 |
| LEAP-Seq n confirming: | 35 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCAAAGTTGTGCAGCTCAT |
| Suggested primer 2: | ACACGAGTTCGGAAGCATGT |