| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.067048 |
| Chromosome: | chromosome 4 |
| Location: | 1964017 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g218850 | RNP | (1 of 1) K14846 - ribosome production factor 1 (RPF1); Putative small nucleolar ribonucleoprotein U3 protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAGTTCTACCCAGTGAGTTCTACCTCATTATGTGTTCCTGCCCCTGCCT |
| Internal bar code: | GTGGATTGCGTTCTGTTAATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 980 |
| LEAP-Seq percent confirming: | 93.4783 |
| LEAP-Seq n confirming: | 43 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACACTAGTCATCTGGCGGG |
| Suggested primer 2: | AATATGCAGGCCCTGGCTTT |