| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.067053 |
| Chromosome: | plastome |
| Location: | 84446 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| CreCp.g802298 | ChreCp033,rpoA,2717004 | (1 of 1) PF03118 - Bacterial RNA polymerase, alpha chain C terminal domain (RNA_pol_A_CTD); RNA polymerase alpha subunit | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAAACAAAAACCTTATAATTTAGATGTGACAACTTTAAAAAAACGCAATA |
| Internal bar code: | GTGGCTTATATGTGTAAAAGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 74 |
| LEAP-Seq percent confirming: | 33.3333 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGAAAGAGCGTCCCCTTC |
| Suggested primer 2: | TCAAAACAACTGCAGCAGCC |