Insertion junction: CLIP2.067061_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.067061
Chromosome:chromosome 16
Location:4051831
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g690350 SNR6 SNARE-associated Golgi protein; (1 of 1) PTHR12677:SF24 - TRANSMEMBRANE PROTEIN 64 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCGCCCAAATGCTGCTGGGAGGTGCGCAGCCCGGCAATACGGACGGACG
Internal bar code:CGGGAAAGTGGTTAGGCATATA

Confirmation - LEAP-Seq

LEAP-Seq distance:1988
LEAP-Seq percent confirming:96.875
LEAP-Seq n confirming:31
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:32

Suggested primers for confirmation by PCR

Suggested primer 1:GTTGGTTGTTGGCCGTGTAC
Suggested primer 2:ACCTACTCCCTACCACGACC