| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.067063 |
| Chromosome: | chromosome 2 |
| Location: | 403685 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g075800 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGCAACTGCTGCTGCCTTCAGCTCGTTCACCACCGGCCTCTCCATGTCA |
| Internal bar code: | TTGTCCGCGGTTGGATCACAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3635 |
| LEAP-Seq percent confirming: | 99.5496 |
| LEAP-Seq n confirming: | 221 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 222 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTAGACACATGCTGCATGC |
| Suggested primer 2: | GAAGCCGTACCAGAGAGCTC |