| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.067068 |
| Chromosome: | chromosome 16 |
| Location: | 1551165 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g653450 | BAR1 | Bin/Amphiphysin/Rvs domain protein 1; (1 of 1) PF03114 - BAR domain (BAR) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTCTTTGCAAGCGCCGAACACAAGAGCAGGCGCCATGGGCTACGGATGG |
| Internal bar code: | TGATGAACATGGCAACATCTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 339 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAACGTGTGAGAGGGCAAGA |
| Suggested primer 2: | ATTGTTGCCTGCTGTGCTTG |