| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.067070 |
| Chromosome: | chromosome 10 |
| Location: | 4723202 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g452400 | CSF1 | Chlamydomonas specific family F protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTTGGATGTATCAAGCGGCAAGTGGCAAGCAGGGCAGGGCATCAATCAC |
| Internal bar code: | GATGTTTTTCACTAAGGGTATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 114 |
| LEAP-Seq percent confirming: | 2.46914 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 79 |
| LEAP-Seq n unique pos: | 81 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCAAGAGAGCTGGACACAT |
| Suggested primer 2: | TTGACGGTGTGCGATCAAGA |