| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.067085 |
| Chromosome: | chromosome 4 |
| Location: | 3283974 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g226811 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATATATGTTACTAGTAGTCTTCACGCCGCCAGCTCCGAGCACCCACATGC |
| Internal bar code: | GTACCTAGCTGTTGTATTTTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1141 |
| LEAP-Seq percent confirming: | 50.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGCCGATGGAAGGGAAGGA |
| Suggested primer 2: | ATTACAGCGTGTGTGCCAGA |