Insertion junction: CLIP2.067094_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.067094
Chromosome:chromosome 2
Location:7302729
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g391393 (1 of 726) IPR011009 - Protein kinase-like domain 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGTGTACAGGTGTGTCAACCACGCACGTGCCAGATACGGGATGAGTATC
Internal bar code:AATGTTGGGCACGTAGGTAGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:4413
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:67
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:67

Suggested primers for confirmation by PCR

Suggested primer 1:TGCGCAACCCTTTGTTAAGC
Suggested primer 2:AGCTAGGGAGGGGGTACAAG