Insertion junction: CLIP2.067097_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.067097
Chromosome:chromosome 1
Location:5346391
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g037650 (1 of 1) PTHR15069//PTHR15069:SF1 - FAMILY NOT NAMED // PROTEASOME ASSEMBLY CHAPERONE 1 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGTCCACCGGGATGACGCTCCTGCTGCAGCCCTCCGCCGGCCAGTCTCAA
Internal bar code:ACTGGCCAGAGGCTTTCTTGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:4377
LEAP-Seq percent confirming:3.75
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:77
LEAP-Seq n unique pos:80

Suggested primers for confirmation by PCR

Suggested primer 1:CGGACTCCACACCTCAATCC
Suggested primer 2:AAGGATGCCGTGACCTTCTG